Dinmukhamed Toishybek
@zool.kz
Laboratory of cryobiology and biobank of germplasm of wild animals of Kazakhstan
Institute zoology
RESEARCH INTERESTS
cryobiology
Scopus Publications
Scopus Publications
Y. Toishibekov, D.Y. Toishybek, T.T. Nurkenov, B.S. Katubayeva, M.Y. Salmenova, and A.V. Perfilyeva
al-Farabi Kazakh National University
The Tobet dog breed, a national heritage of Kazakhstan, is threatened with extinction and conservation measures are urgently needed. This study presents a pioneering approach to preserve the genetic diversity and survival of this endangered breed through cryopreservation and cryobanking. We have optimised methods for cryopreservation of somatic cells (fibroblasts) isolated from skin explants of Tobet dogs and investigated the effects of in vitro cultivation on cell viability. Our results emphasise the importance of selecting appropriate culture media and cryoprotectants. Dulbecco's Modified Eagle Medium (DMEM) and ethylene glycol (EG) were found to be the most effective agents for increasing the growth rate and viability of fibroblasts after thawing. Through careful experimentation, including the evaluation of equilibrium and non-equilibrium cryopreservation techniques and the application of various cryoprotectants, we have established the first Tobet cryobank for somatic cells. The establishment of a cryobank represents a significant step towards the conservation of the Tobet dog breed and provides a model for the conservation of other endangered species. This study not only contributes to the preservation of Kazakhstan's cultural and biological heritage, but also opens up new avenues for the application of biotechnological approaches to wildlife conservation.
Timur Saliev, Ildar Fakhradiyev, Shynar Tanabayeva, Yelena Assanova, Dinmukhamed Toishybek, Aigul Kazybayeva, Baimakhan Tanabayev, Marat Sikhymbaev, Aliya Alimbayeva, and Yerzhan Toishibekov
Informa UK Limited
Abstract Background The negative effects of ionizing radiation on organs and the reproductive system are well known and documented. Exposure to gamma radiation can lead to oligospermia, azoospermia and DNA damage. Up to date, there is no effective pharmaceutical compound for protecting the male reproductive system and sperm. Objective This study aimed at investigating the ability of Ɛ-aminocaproic acid (EACA) to prevent the damage of human spermatozoa and DNA induced by ionizing radiation. Materials and methods Sperm samples were obtained from healthy volunteers (35 men; 31.50 ± 7.34 years old). There were four experimental groups: (1) control group (CG), (2) group exposed to maximal radiation dose 67.88 mGy (RMAX), (3) low-dose radiation (minimal) 22.62 mGy (RMIN), and (4) group treated with radiation (67.88 mGy) and EACA (dose 50 ng/mL). Sperm motility, viability, and DNA damage were assessed. Results We observed a significant decrease in total sperm motility of the RMAX group compared to CG (p < .05). Sperm viability in the RMAX group was also reduced in comparison to the control (p < .05). A significant increase in DNA fragmentation was detected in the RMAX group. The results demonstrated that the treatment of sperm with EACA led to a decrease in the fragmentation of the sperm DNA (compared to the RMAX group) (p < .05). Conclusion The results indicate that EACA effectively protects human spermatozoa from DNA damage induced by ionizing radiation. Treatment of spermatozoa with EACA led to the preservation of cell motility, viability, and DNA integrity upon radiation exposure.
Y. M. Toishibekov, A. S. Kazybayeva, Y. A. Assanova, T. T. Nurkenov, and D. Y. Toishybek
CSIRO Publishing
A.S. Seisenbayeva, V. Isachenko, Y.A. Assanova, X.X. Du, D.Y. Toishybek, and Y.M. Toishibekov
Elsevier BV