NASIM AHMAD YASIN
@pu.edu.pk
Associate Professor, Horticulture
University of the Punjab
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Horticulture, Agronomy and Crop Science, Multidisciplinary
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Adil Dilawar, Iqra Shahzadi, Nasim Ahmad Yasin, Faheem Adil, Shafaq Sahar, Mohd Tanveer Alam Khan, Waheed Akram, and Aqeel Ahmad
CRC Press
Areeba Rehman, Waheed Akram, Tehmina Anjum, Nasim Ahmad Yasin, and Zill e Huma Aftab
CRC Press
Imran Khan, Areeba Rehman, Waheed Akram, Tehmina Anjum, Nasim Ahmad Yasin, Zill-e-Huma Aftab, Bareera Munir, Waheed Ullah Khan, and Guihua Li
MDPI AG
Salinity stress poses a severe risk to food security and crop productivity. Stress reduction techniques are not necessarily sustainable or environmentally friendly. With the increasing adverse impact of salinity and area, it is necessary to restore and ameliorate salinity stress using environmentally friendly approaches. In this context, beneficial rhizospheric microbes may offer a sustainable approach to managing salinity stress. We used Bacillus subtilis strain Z-12 and B. aryabhattai strain Z-48 to improve the growth of turnip (Brassica rapa subsp. rapa) plants under salinity stress conditions and elucidated the beneficial impact of these bacterial strains on different physiological and biochemical aspects of plants. The application of both strains had a significant (p < 0.05) positive influence on analyzed parameters under salt stress. Here, B. aryabhattai strain Z-48 superiorly increased shoot length (33.2-, 25.8%), root length (38.6-, 31.5%), fresh biomass (23.9-, 17.8%), and dry biomass (38.60-, 48.6%) in normal and saline stress (200 mM NaCl) conditions, respectively. Physiological studies showed that antioxidant enzyme activities were significantly increased by B. subtilis Z-12 and B. aryabhattai Z-48 under salinity stress, with a few exceptions. Moreover, the inoculation of both strains effectively increased total chlorophyll, soluble sugar, phenolic, flavonoid, and glucosinolate contents under simulated salinity stress and normal conditions. Hence, these findings support the framework that inoculating turnip plants with these strains can enhance their tolerance against salinity stress.
Mahrukh Haroon, Waheed Ullah Khan, Bareera Munir, Sajid Rashid Ahmad, Areeba Rehman, Waheed Akram, Awais Munir, Rehana Sardar, and Nasim Ahmad Yasin
Elsevier BV
Muhammad Sajid, Shakil Ahmed, Rehana Sardar, Aamir Ali, and Nasim Ahmad Yasin
PeerJ
The continuous contamination of heavy metals (HMs) in our ecosystem due to industrialization, urbanization and other anthropogenic activities has become a serious environmental constraint to successful crop production. Lead (Pb) toxicity causes ionic, oxidative and osmotic injuries which induce various morphological, physiological, metabolic and molecular abnormalities in plants. Polyethylene glycol (PEG) is widely used to elucidate drought stress induction and alleviation mechanisms in treated plants. Some recent studies have unveiled the potential of PEG in regulating plant growth and developmental procedures including seed germination, root and shoot growth and alleviating the detrimental impacts of abiotic stresses in plants. Therefore, the current study aimed to assess the effects of seed priming with various concentrations (10%, 20%, 30% and 40%) of PEG on the growth and development of radish plants growing under Pb stress (75 mg/kg soil). Lead toxicity reduced root growth (32.89%), shoot growth (32.81%), total chlorophyll (56.25%) and protein content (58.66%) in treated plants. Similarly, plants showed reduced biomass production of root (35.48%) and shoot (31.25%) under Pb stress, while 30% PEG seed priming enhanced biomass production of root (28.57%) and shoot (35.29%) under Pb contaminated regimes. On the other hand, seedlings obtained from 30% PEG priming demonstrated a notable augmentation in the concentrations of photosynthetic pigments, antioxidative activity and biomass accumulation of the plants. PEG-treated plants showed modulations in the enzymatic activities of peroxidase (PO), catalase (CAT) and superoxide dismutase (SOD). These changes collectively played a role in mitigating the adverse effects of Pb on plant physiology. Our data revealed that PEG interceded stress extenuation encompasses numerous regulatory mechanisms including scavenging of ROS through antioxidant and non-antioxidants, improved photosynthetic activity and appropriate nutrition. Hence, it becomes necessary to elucidate the beneficial role of PEG in developing approaches for improving plant growth and stress tolerance.
Saba Mudassar, Shakil Ahmed, Rehana Sardar, Nasim Ahmad Yasin, Muhammad Jabbar, and Maximilian Lackner
MDPI AG
Cadmium (Cd) is one of the foremost phytotoxic elements. Its proportion in agricultural soil is increasing critically due to anthropogenic activities. Cd stress is a major crop production threat affecting food security globally. Triacontanol (TRIA) is a phytohormone that promotes growth, development, and metabolic processes in plants. The current study explicates the mitigation of Cd toxicity in Vigna radiata L. (mung bean) seedlings through the application of TRIA by a seed priming technique under Cd stress. The role of TRIA in improving metabolic processes to promote Vigna radiata (mung bean, green gram) vegetative growth and performance under both stressed and unstressed conditions was examined during this study. To accomplish this, three doses of TRIA (10, 20, and 30 µmol L−1) were used to pretreat V. radiata seeds before they were allowed to grow for 40 days in soil contaminated with 20 mg kg−1 Cd. Cd stress lowered seed germination, morphological growth, and biomass in V. radiata plants. The maximum root and shoot lengths, fresh and dry weights of roots, and shoot and seed germination rates were recorded for TRIA2 compared with those of TRIA1 and TRIA3 under Cd stress. In Cd-stressed V. radiata plants, TRIA2 increased the content of chlorophyll a (2.1-fold) and b (3.1-fold), carotenoid (4.3-fold), total chlorophyll (3.1-fold), and gas exchange attributes, such as the photosynthetic rate (2.9-fold), stomatal conductance (6.0-fold), and transpiration rate (3.5-fold), compared with those in plants treated with only Cd. TRIA seed priming increased nutrient uptake (K1+, Na1+, Mg2+, and Zn2+), total phenolic content, total soluble protein content, and DPPH (2,2-diphenyl-1-picrylhydrazyl) activity. Additionally, TRIA2 significantly reduced the quantity of Cd in the plants (3.0-fold) and increased the metal tolerance index (6.6-fold) in plants contrasted with those in the Cd-treated plants. However, TRIA2 promoted plant growth and biomass production by lowering Cd-induced stress through modifying the plant antioxidant machinery and reducing oxidative stress. The improved yield characteristics of V. radiata seedlings treated with TRIA suggest that exogenous TRIA may be used to increase plant tolerance to Cd stress.
Shakil Ahmed, Aleeza Akram, Rehana Sardar, Nasim Ahmad Yasin, Mariam Fatima, Muhammad Jabbar, and Maximilian Lackner
MDPI AG
Agricultural soil contaminated with heavy metals gradually affects crop yield and its quality. Cadmium (Cd) is a heavy metal that severely affects crop yield, such as Brassica rapa L. (turnip), which is grown in arid and semiarid regions worldwide. It also affects seed germination and seedling development. The exogenous application of triacontanol (Tria, C30H61OH) has the potential to alleviate heavy metal-induced toxic effects and promote crop yield even in contaminated environments. Therefore, in the present work, Tria was tested to lessen the toxicity of Cd to turnip plants. The current study aimed to determine how seed priming and foliar application of Tria (10 and 20 ppm) influence the morphophysiological and yield characteristics of turnip plants under Cd-induced growth inhibition. Cd reduced turnip growth by affecting its morphology, biomass, and yield parameters. On the other hand, Tria at 20 ppm via SP+FS (seed priming + foliar spray) enhanced plant growth by increasing its root and leaf fresh weight by 80 and 54%, Chl a (59%), Chl b (27%), phenolic content (39%), and mineral contents of Mg (60%) and K (39%) compared with those in the plants treated with only Cd. DPPH (2,2-diphenyl-1-picrylhydrazyl) activity was enhanced by up to 48% and ascorbic acid content by up to 96% in Cd-treated plants. These findings suggest that Tria application via both methods improved turnip yield by increasing tolerance to Cd toxicity. Therefore, this study paves the way for further exploration into a very cheap and economical way of enhancing crop production against Cd stress for farmers.
Shakil Ahmed, Mariam Fatima, Rehana Sardar, and Nasim Ahmad Yasin
Springer Science and Business Media LLC
Anis Ali Shah, Nasim Ahmad Yasin, Muhammad Ahsan Altaf, and Aqeel Ahmad
Elsevier BV
Saber Hussain, Shakil Ahmed, Waheed Akram, Aqeel Ahmad, Nasim Ahmad Yasin, Mei Fu, Guihua Li, and Rehana Sardar
Elsevier BV
Shakil Ahmed, Mehtab Qasim, Rehana Sardar, Nasim Ahmad Yasin, and Ismat Umar
Informa UK Limited
High fluoride (F) concentrations negatively affect the seed germination, plant growth, development, and yield of crops. Phaseolus vulgaris L. is an F-sensitive crop frequently grown on marginal lands affected by F salts. Selenium (Se) is a vital elicitor of the antioxidative enzymes involved in scavenging free radicals to alleviate abiotic stress. Recent studies have demonstrated that engineered nanoparticles (NPs) have the potential to induce tolerance to abiotic stress in plants. Phytosynthesis of NPs is a novel and sustainable approach to mitigate abiotic stresses. The present study was intended to assess the role of green synthesized Se-nanoparticles (Se-NPs) in improving the physiochemical attributes, growth, and F stress tolerance of P. vulgaris growing in 200 ppm sodium fluoride (NaF) stress. NaF toxicity reduced Chl a, Chl b, and carotenoid content by 88.8%, 95.5%, and 96% compared to control with maximum improvement obtained through phyto-nano seed priming and foliar spray of 70 ppm Se-NPs. The joint treatment of NPs application through seed priming and foliar spray improved stomatal conductance (14.2%) and transpiration rate (11.7%) in plants subjected to NaF stress. The protein content (91.02%) and DPPH activity (33.72%) decreased under NaF stress, which was improved by phyto-nano seed priming and foliar spray (14.10%). Furthermore, the integrated application of Se-NPs seed priming and foliar spray increased nutritional content (P, K, Ca, Mg, and Zn), proline, ascorbic acid, and phenol yet reduced the level of NaF in plants. Se-NPs at 70 ppm were found to be more effective than 60 ppm in all modes of applications. Our results reveal a perception that Se-NPs increase P. vulgaris growth in NaF stress conditions, perhaps through a multipronged approach: improving photosynthetic content, nutrient uptake, and yield of P. vulgaris. Consequently, the findings of this study may be used for breeding and screening F-tolerant cultivars.
Shakil Ahmed, Sana Ashraf, Nasim Ahmad Yasin, Rehana Sardar, Ibrahim Al-Ashkar, Magdi T. Abdelhamid, and Ayman El Sabagh
Informa UK Limited
The increasing levels of cadmium (Cd) pollution in agricultural soil reduces plant growth and yield. This study aims to determine the impact of green synthesized zinc oxide nanoparticles (ZnO-NPs) on the physiochemical activities, nutrition, growth, and yield of Zea mays L. under Cd stress conditions. For this purpose, ZnO-NPs (450 ppm and 600 ppm) synthesized from Syzygium aromaticum were applied through foliar spray to Z. mays and also used as seed priming agents. A significant decline in plant height (35.24%), biomass production (43.86%), mineral content, gas exchange attributes, and yield (37.62%) was observed in Cd-spiked plants compared to the control. While, 450 ppm ZnO-NPs primed seed increased plant height (18.46%), total chlorophyll (80.07%), improved ascorbic acid (25.10%), DPPH activity (26.66%), and soil mineral uptake (Mg+2 (38.86%), K+ (27.83%), and Zn+2 (43.68%) as compared to plants only spiked with Cd. On the contrary, the foliar-applied 450 ppm ZnO-NPs increased plant height (8.22%), total chlorophyll content (73.59%), ascorbic acid (21.39%), and DPPH activity (17.61%) and yield parameters; cob diameter (19.45%), and kernels numbers 6.35% enhanced compared to plants that were spiked only with Cd. The findings of the current study pave the way for safer and more cost-effective crop production in Cd-stressed soils by using green synthesized NPs and provide deep insights into the underlying mechanisms of NPs treatment at the molecular level to provide compelling evidence for the use of NPs in improving plant growth and yield.
Aqeel Ahmad, Waheed Akram, Rehana Sardar, and Nasim Ahmad Yasin
Frontiers Media SA
Hafiz Zulqurnain Raza, Anis Ali Shah, Zahra Noreen, Sheeraz Usman, Sadia Zafar, Nasim Ahmad Yasin, Shaban R.M. Sayed, Fahed A. Al-Mana, Hosam O. Elansary, Aqeel Ahmad,et al.
Elsevier BV
Saber Hussain, Shakil Ahmed, Waheed Akram, Rehana Sardar, Muhammad Abbas, and Nasim Ahmad Yasin
Informa UK Limited
Salt toxicity is one of the foremost environmental stresses that declines nutrient uptake, photosynthetic activity and growth of plants resulting in a decrease in crop yield and quality. Seed priming has become an emergent strategy to alleviate abiotic stress and improve plant growth. During the current study, turnip seed priming with sodium selenite (Na2SeO3) was investigated for its ability to mitigate salt stress. Turnip (Brassica rapa L. var. Purple Top White Globe) seeds primed with 75, 100, and 125 μML-1 of Se were subjected to 200 mM salt stress under field conditions. Findings of the current field research demonstrated that salt toxicity declined seed germination, chlorophyll content, and gas exchange characteristics of B. rapa seedling. Whereas, Se-primed seeds showed higher germination rate and plant growth which may be attributed to the decreased level of hydrogen peroxide (H2O2) and malondialdehyde (MDA) decreased synthesis of proline (36%) and besides increased total chlorophyll (46%) in applied turnip plants. Higher expression levels of genes encoding antioxidative activities (CAT, POD, SO,D and APX) mitigated oxidative stress induced by the salt toxicity. Additionally, Se treatment decreased Na+ content and enhanced K+ content resulting in elevated K+/Na+ ratio in the treated plants. The in-silico assessment revealed the interactive superiority of Se with antioxidant enzymes including CAT, POD, SOD, and APX as compared to sodium chloride (NaCl). Computational study of enzymes-Se and enzymes-NaCl molecules also revealed the stress ameliorative potential of Se through the presence of more Ramachandran-favored regions (94%) and higher docking affinities of Se (-6.3). The in-silico studies through molecular docking of Na2SeO3, NaCl, and ROS synthesizing enzymes (receptors) including cytochrome P450 (CYP), lipoxygenase (LOX), and xanthine oxidase (XO), also confirmed the salt stress ameliorative potential of Se in B. rapa. The increased Ca, P, Mg, and Zn nutrients uptake nutrients uptake in 100 μML-1 Se primed seedlings helped to adjust the stomatal conductivity (35%) intercellular CO2 concentration (32%), and photosynthetic activity (41%) resulting in enhancement of the yield attributes. More number of seeds per plant (6%), increased turnip weight (115 gm) root length (17.24 cm), root diameter (12 cm) as well as turnip yield increased by (9%tons ha-1) were recorded for 100 μML-1 Se treatment under salinity stress. Findings of the current research judiciously advocate the potential of Se seed priming for salt stress alleviation and growth improvement in B. rapa.
Naima Huma Naveed, Naveed Abbas Nasir, Anis Ali Shah, Tahir Shahzad, Nasim Ahmad Yasin, Zain Ali, Muhammad Jamil, Talha Javed, and Aamir Ali
Elsevier BV
Ahmad Jamal, Nasim Ahmad Yasin, Sumera Javad, Shakil Ahmed, Ayesha Yasmin, Ozair Chaudhry, Mohamed Saad Daoud, and Mansour K. Gatasheh
Elsevier BV
Shakil Ahmed, Saba Mudassar, Rehana Sardar, and Nasim Ahmad Yasin
Springer Science and Business Media LLC
Ismat Umar, Shakil Ahmed, Nasim Ahmad Yasin, Abdul Wahid, Saud Alamri, Yasir Hamid, and Rehana Sardar
Elsevier BV
Mawra Khan, Shakil Ahmed, Nasim Ahmad Yasin, Rehana Sardar, Muhammad Hussaan, Abdel-Rhman Z. Gaafar, and Faish Ullah Haider
MDPI AG
Brassinosteroids (BRs) influence a variety of physiological reactions and alleviate different biotic and abiotic stressors. Turnip seedlings were grown with the goal of further exploring and expanding their function in plants under abiotic stress, particularly under heavy metal toxicity (lead stress). This study’s objective was to ascertain the role of applied 28-homobrassinolide (HBL) in reducing lead (Pb) stress in turnip plants. Turnip seeds treated with 1, 5, and 10 µM HBL and were grown-up in Pb-contaminated soil (300 mg kg−1). Lead accumulation reduces biomass, growth attributes, and various biochemical parameters, as well as increasing proline content. Seed germination, root and shoot growth, and gas exchange characteristics were enhanced via HBL treatment. Furthermore, Pb-stressed seedlings had decreased total soluble protein concentrations, photosynthetic pigments, nutrition, and phenol content. Nonetheless, HBL increased chlorophyll a and chlorophyll b levels in plant, resulting in increased photosynthesis. As a result, seeds treated with HBL2 (5 µM L−1) had higher nutritional contents (Mg+2, Zn+2, Na+2, and K+1). HBL2-treated seedlings had higher DPPH and metal tolerance indexes. This led to the conclusion that HBL2 effectively reduced Pb toxicity and improved resistance in lead-contaminated soil.
Saber Hussain, Shakil Ahmed, Nasim Ahmad Yasin, Waheed Akram, Rehana Sardar, Aqeel Ahmad, and Guihua Li
Elsevier BV
Maria Ahmad, Shakil Ahmed, Nasim Ahmad Yasin, Abdul Wahid, and Rehana Sardar
Springer Science and Business Media LLC
Muhammad Akbar, Ali Raza, Tayyaba Khalil, Nasim Ahmad Yasin, Yasir Nazir, and Aqeel Ahmad
Elsevier BV
Saber Hussain, Shakil Ahmed, Waheed Akram, Guihua Li, and Nasim Ahmad Yasin
Frontiers Media SA
Various abiotic stresses may affect the germination, growth, and yield of direct-seeded vegetable crops. Seed priming with effective antioxidant mediators may alleviate these environmental stresses by maintaining uniformity in seed germination and improving the subsequent health of developing seedlings. Salt-induced stress has become a limiting factor for the successful cultivation of Brassica rapa L., especially in Southeast Asian countries. The present study was performed to elucidate the efficacy of seed priming using selenium (Se) in mitigating salt-induced oxidative stress in turnip crops by reducing the uptake of Na+. In this study, we administered three different levels of Se (Se-1, 75 μmol L−1; Se-2, 100 μmol L−1; and Se-3, 125 μmol L−1) alone or in combination with NaCl (200 mM). Conspicuously, salinity and Se-2 modulated the expression levels of the antioxidant genes, including catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and ascorbate peroxidase (APX). The upregulated expression of stress-responsive genes alleviated salt stress by scavenging the higher reactive oxygen species (ROS) level. The stress ameliorative potential of Se (Se-2 = 100 μmol L−1) enhanced the final seed germination percentage, photosynthetic content, and seedling biomass production up to 48%, 56%, and 51%, respectively, under stress. The advantageous effects of Se were attributed to the alleviation of salinity stress through the reduction of the levels of malondialdehyde (MDA), proline, and H2O2. Generally, treatment with Se-2 (100 μmo L−1) was more effective in enhancing the growth attributes of B. rapa compared to Se-1 (75 μmo L−1) and Se-3 (125 μmo L−1) under salt-stressed and non-stressed conditions. The findings of the current study advocate the application of the Se seed priming technique as an economical and eco-friendly approach for salt stress mitigation in crops grown under saline conditions.