Lyazzat Mukhangaliyeva
@nu.edu.kz
Scopus Publications
Scopus Publications
Lyazzat Mukhangaliyeva, Samed Kocer, Alkris Warren, Kevan Bell, Marian Boktor, Mustafa Yavuz, Eihab Abdel-Rahman, and Parsin Haji Reza
Optica Publishing Group
Optically shifting the focal plane to allow depth scanning of delicate biological structures and processes in their natural environment offers an appealing alternative to conventional mechanical scanning. Our technique uses a deformable mirror-based photoacoustic remote sensing microscopy (PARS) with a focus shifting of Δz ∼ 240 µm. We achieve this by integrating a deformable mirror that functions as a varifocal mirror for axial scanning. First, the system's focal shift capability was demonstrated with USAF resolution targets and carbon fiber phantoms, followed by in-vivo visualizations of blood vessels in chicken embryo chorioallantoic membrane (CAM). This work represents an initial step toward developing a non-contact, label-free, and aberration-free PARS imaging system with axial scanning capability.
Amr Kamel, Samed Kocer, Lyazzat Mukhangaliyeva, Resul Saritas, Ahmet Gulsaran, Alaa Elhady, Mohamed Basha, Parsin Hajireza, Mustafa Yavuz, and Eihab Abdel-Rahman
MDPI AG
A novel MEMS continuous deformable mirror (DM) is presented. The mirror can be integrated into optical systems to compensate for monochromatic and chromatic aberrations. It is comprised of a 1.6 mm circular plate supported by eight evenly spaced flexural springs. Unlike traditional bias actuated DMs, it uses resonant electrostatic actuation (REA) to realize low- and high-order Zernike modes with a single drive signal. Instead of the hundreds or thousands of electrodes deployed by traditional DMs, the proposed DM employs only 49 electrodes and eliminates the need for spatial control algorithms and associated hardware, thereby providing a compact low-cost alternative. It also exploits dynamic amplification to reduce power requirements and increase the stroke by driving the DM at resonance. The DM was fabricated using a commercial silicon-on-insulator (SOI) MEMS process. Experimental modal analysis was carried out using laser Doppler vibrometry (LDV) to identify mode shapes of the DM and their natural frequencies. We are able to observe all of the lowest eight Zernike modes.
Samed Kocer, Lyazzat Mukhangaliyeva, Resul Saritas, Ahmet Gulsaran, Alaa Elhady, Kevan Bell, Amr Kamel, Mohamed Basha, Taylan Das, Muhammed Kayaharman,et al.
IEEE
This paper presents a novel varifocal mirror made of a micro-electromechanical system (MEMS) continuous deformable mirror (DM). It uses resonant electrostatic actuation (REA) via single voltage waveform to vary the focus of a 1.6 mm circular DM. Unlike traditional DMs, REA eliminates the need for individually addressable electrodes and the use of complex influence functions. It also minimizes the electrode count, thereby providing for simpler and faster modulating DMs. The DM was integrated into an adaptive optics system as varifocal mirror. The system was able to realize an 8 cm focal shift between focal lengths of 11 cm and 19 cm by changing the phase angle between the DM drive signal and the pulse signal of the incident laser beam.
Zohreh Hosseinaee, Nima Abbasi, Nicholas Pellegrino, Layla Khalili, Lyazzat Mukhangaliyeva, and Parsin Haji Reza
Springer Science and Business Media LLC
AbstractEarly diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for simultaneous, non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.
Zohreh Hosseinaee, Benjamin Ecclestone, Nicholas Pellegrino, Layla Khalili, Lyazzat Mukhangaliyeva, Paul Fieguth, and Parsin Haji Reza
Optica Publishing Group
Stimulated Raman scattering (SRS) has been widely used in functional photoacoustic microscopy to generate multiwavelength light and target multiple chromophores inside tissues. Despite offering a simple, cost-effective technique with a high pulse repetition rate; it suffers from pulse-to-pulse intensity fluctuations and power drift that can affect image quality. Here, we propose a new technique to improve the temporal stability of the pulsed SRS multiwavelength source. We achieve this by lowering the temperature of the SRS medium. The results suggest that a decrease in temperature causes an improvement of temporal stability of the output, considerable rise in the intensity of the SRS peaks, and significant increase of SRS cross section. The application of the method is shown for in vivo functional imaging of capillary networks in a chicken embryo chorioallantois membrane using photoacoustic remote sensing microscopy.
Kevan Bell, Lyazzat Mukhangaliyeva, Layla Khalili, and Parsin Haji Reza
Optica Publishing Group
An improved method of remote optical absorption spectroscopy and hyperspectral optical absorption imaging is described which takes advantage of the photoacoustic remote sensing detection architecture. A wide collection of photoacoustic excitation wavelengths ranging from 210 nm to 1550 nm was provided by a nanosecond tunable source allowing access to various salient endogenous chromophores such as DNA, hemeproteins, and lipids. Sensitivity of the device was demonstrated by characterizing the infrared absorption spectrum of water. Meanwhile, the efficacy of the technique was explored by recovering cell nuclei and oxygen saturation from a live chicken embryo model and by recovering adipocytes from freshly resected murine adipose tissue. This represents a continued investigation into the characteristics of the hyperspectral photoacoustic remote sensing technique which may represent an effective means of non-destructive endogenous contrast characterization and visualization.
Amr Kamel, Samed Kocer, Taylan Das, Lyazzat Mukhangaliyeva, Resul Saritas, Parsin Hajireza, Mustafa Yavuz, and Eihab Abdel-Rahman
IEEE
We present a novel continuous deformable mirror (DM). The mirror can be integrated into optical systems to compensate for multiple wavefront aberrations during real-time scanning of the human retina and other targets. Specifically, it can correct all seven of the wavefront aberrations dominant in ophthalmic applications. The mirror assumes the desired shape to correct an aberration via resonant electrostatic actuation. It eliminates the need for a large number of electrodes and dependence algorithm required for spatial control of segmented DMs. It also realizes an additional benefit of dynamic amplification, thereby increasing the stroke distance. In addition, we demonstrate that the fundamental (defocus) mode of the DM can be used to change the location of the focal point for a reflected laser beam by up to $485\\ \\mu\\mathrm{m}$.
Kevan Bell, Lyazzat Mukhangaliyeva, Layla Khalili, and Parsin Haji Reza
SPIE
A hyperspectral photoacoustic remote sensing microscope is used to investigate and image optical absorption contrast in live and resected chicken tissues ranging from 250 nm to 1210 nm highlighting DNA, hemeproteins, and lipids.