Beatriz Bertolaccini Martinez (Portuo)
@univas.edu.br
Programa de Pós-graduação Profissional em Ciências Aplicadas à Saúde
Universidade do Vale do Sapucaí
RESEARCH, TEACHING, or OTHER INTERESTS
General Medicine
Scopus Publications
Scopus Publications
Beatriz Bertolaccini Martínez, Karoline Maria Moraes da Silva, Fernanda Moraes Ramos, Gabriel Brandão da Costa, Ana Júlia Pereira de Oliveira, and Solange Ribeiro Moraes
EDIPUCRS
Introdução: na atenção primária à saúde, a atuação de médicos generalistas é importante no rastreamento e no acompanhamento dos estágios iniciais da doença renal crônica devido à alta prevalência, à falta de especialistas, ao impacto na morbimortalidade e qualidade de vida e ao elevado custo aos sistemas de saúde. O uso de aplicativos móveis tornou-se importante na medicina contemporânea por facilitar o acesso à informação, o que otimiza a prestação de cuidados à saúde. Objetivos: desenvolver e validar um aplicativo móvel sobre testes laboratoriais na doença renal crônica para médicos generalistas. Métodos: estudo na modalidade de produção tecnológica, com desenvolvimento de software a partir de revisão da literatura, com validação por especialistas (nefrologistas) e por médicos generalistas. A validação se deu através de respostas a questionários, que avaliaram conteúdo, compreensão, linguagem e apresentação gráfica do aplicativo. Para a análise da consistência interna dos questionários foi utilizado o coeficiente alfa de Cronbach e para avaliar a concordância dos médicos sobre aspectos do aplicativo utilizou-se o Índice de Validade de Conteúdo. O valor adotado para o Índice de Validade de Conteúdo foi maior do que 80%. Resultados: as respostas dos nefrologistas (n=9) e dos médicos generalistas (n=13) foram, respectivamente, medianas de idade de 40 (35-71) anos e 45 (30-50) anos; sexo feminino 5 (55%) e 7 (54%); coeficiente alfa de Cronbach de 0,8025 e 0,9145; Índice de Validade de Conteúdo Global de 95,6% e 92,1%. Conclusão: a confiabilidade das respostas aos questionários mostrou-se boa e houve excelente concordância entre os juízes sobre os aspectos do aplicativo móvel.
Beatriz Bertolaccini Martínez, Ana Carolina Couto Pereira, Júlio Henrique Muzetti, Fernando de Paiva Telles, Fiorita Gonzáles Lopes Mundim, and Manoel Araújo Teixeira
FapUNIFESP (SciELO)
PURPOSE:
To evaluate metabolic effects in experimental model of glucocorticoid-induced insulin resistance.
METHODS:
Twenty Wistar male rats were randomly divided into two groups, which were treated with intraperitoneally injected dexamethasone 1mg/Kg/day for ten days consecutively (Group D; n=10) and placebo (Group C; n=10). The variables analyzed were: from the first to the 10th day - body weight (before and after treatment); food and water daily consumption; on the 10th day - glycemia, insulinemia, HOMA-beta and HOMA-IR. The blood samples for laboratory analysis were obtained by intracardiac puncture. Also on the 10th day liver fragments were taken for analyzing glycogen and fattty.
RESULTS:
Group D animals compared to group C had: weight reduction (g), (D=226.5±24.7 vs C=295.0±25.4; p=0.001); increased glycemia (mmol/l) (D=19.5±2.1 vs C=14.2±3.1; p=0.0001); diminished insulinemia (mU/l) (D=0.2±0.1 vs C=2.0±0.4; p=0.0001); reduced HOMA-β (D=0.2±0.1 vs C=4.2±1.7; p=0.0002); diminished HOMA-IR (D=0.2±0.1 vs C=1.3±0.4; p=0.0002). Histological examination of the liver showed that 100% of group D and none of group C had moderate fatty. (p=0.2).
CONCLUSION:
Animals treated with glucocorticoid, in this experimental model, expressed hyperglycemia, hypoinsulinism and decreased peripheral insulin sensitivity.
Adriana Teresa Silva, Antônio José Bernandes Carvalho, Marília Fernandes Andrades, Ruanito Calixo Junior, Miqueline Pivoto Farias Dias, Andréia Maria Silva, Beatriz Bertolaccini Martinez, and Donizeti Cesar Honorato
Mark Allen Group
Aim:The aim of this study was to investigate the effect of vibration training on plantar impression and motor function in patients affected by stroke.Methods:The sample consisted of 28 individuals with hemiparesis after stroke who were randomly assigned to the intervention group (n=18) and the control group (n=10). The instruments used for evaluation were the mini-mental state examination, photopodoscopy and the 6-minute walk test. For treatment, whole body vibration training was used three times a week for 8 weeks. The treatment was carried out in two phases. The first phase, which lasted for 4 weeks, consisted of four sets of exercises with 60 seconds of vibration. In the first exercise, the participants were in a static standing position, feet apart with knees flexed at 30°. In the second exercise, the same position was implemented, with knees flexed at 90°. In the third exercise, a standing position with one-leg support on the affected limb with flexed knee at 30°. The fourth exercise was a repetition of the first exercise. The second phase also lasted for 4 weeks. It consisted of the same exercises but the amount of sets of exercises was doubled.Results:Data were analysed by independent t-test and repeated measures ANOVA with two factors. There was no intergroup nor intragroup statistical difference (P=0.05) in the plantar impression area on the affected and unaffected side; there was only intragroup statistical differences in the 6-minute walk test (P=0.03).Conclusions:Whole-body vibration training did not influence the increase of the plantar impression area and motor function in stroke patients.
Sávia Perina Portilho Falci, Manoel Araujo Teixeira, Pablo Ferreira das Chagas, Beatriz Bertolaccini Martinez, Ana Beatriz Alkmim Teixeira Loyola, Lydia Masako Ferreira, and Daniela Francescato Veiga
FapUNIFESP (SciELO)
PURPOSE
To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against Staphylococcus aureus isolates obtained from lower limb wounds and resistant to several antibiotics.
METHODS
A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the Minimum Inhibitory Concentration (MIC). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0.0125 and 0.00625%. The 12th and 13th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37ºC for 18 hours. The Minimum Bactericidal Concentration (MBC), able of killing all the microorganisms, was also determined. Two S. aureus isolates were obtained from lower limb wounds of female patients and the identification of the microorganisms (Staphylococcus aureus) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan(r). After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the MIC and MBC.
RESULTS
The MIC was 0.2% and the MBC was 0.4%.
CONCLUSION
The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from lower limb wounds which were resistant to multiple antibiotics.
Sávia Perina Portilho Falci, Manoel Araujo Teixeira, Pablo Ferreira das Chagas, Beatriz Bertolaccini Martinez, Ana Beatriz Alkmim Teixeira Loyola, Lydia Masako Ferreira, and Daniela Francescato Veiga
FapUNIFESP (SciELO)
PURPOSE
To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against Staphylococcus aureus isolates obtained from lower limb wounds and resistant to several antibiotics.
METHODS
A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the Minimum Inhibitory Concentration (MIC). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0,0125 and 0.00625%. The 12th and 13th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37ºC for 18 hours. The Minimum Bactericidal Concentration (MBC), able of killing all the microorganisms, was also determined. Two S. aureus isolates were obtained from lower limb wounds of female patients and the identification of the microorganisms (Staphylococcus aureus) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan(r). After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the MIC and MBC.
RESULTS
The MIC was 0.2% and the MBC was 0.4%.
CONCLUSION
The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from lower limb wounds which were resistant to multiple antibiotics.
Beatriz Bertolaccini Martinez and Rodrigo Pereira Custodio
FapUNIFESP (SciELO)
CONTEXT AND OBJECTIVE: The stress of living with a terminal disease has a negative impact on the mental health of hemodialysis (HD) patients. Spirituality is a potential coping mechanism for stressful experiences. Studies on the relationship between spirituality and mental health among HD patients are scarce. The purpose of this study was to evaluate the relationship between mental health and spiritual well-being among HD patients. DESIGN AND SETTING: Cross-sectional observational study on hemodialysis patients at a single center in Brazil, between January and December 2011. METHODS : Mental health was assessed using the General Health Questionnaire and spiritual wellbeing was assessed using the Spiritual Wellbeing Scale; 150 HD patients participated in the study. RESULTS : A significant correlation was found between mental health and spiritual wellbeing (P = 0.001). Spiritual wellbeing was the strongest predictor of mental health, psychological distress, sleep disturbance and psychosomatic complaints. CONCLUSION: Poor mental health was associated with lower spiritual wellbeing. This has important implications for delivery of palliative care to HD patients.
Adriana Teresa Silva, Miqueline Pivoto Faria Dias, Ruanito Calixto, Antonio Luis Carone, Beatriz Bertolaccini Martinez, Andreia Maria Silva, and Donizeti Cesar Honorato
Ovid Technologies (Wolters Kluwer Health)
ObjectiveThe aim of this study was to investigate the acute effects of whole-body vibration on the motor function of patients with stroke. DesignThe present investigation was a randomized clinical trial studying 43 individuals with hemiparesis after stroke, with 33 subjects allocated to the intervention group and 10 subjects allocated to the control group. The intervention group was subjected to one session of vibration therapy (frequency of 50 Hz and amplitude of 2 mm) comprising four 1-min series with 1-min rest intervals between series in three body positions: bipedal stances with the knees flexed to 30 degrees and 90 degrees and a unipedal stance on the paretic limb. The analytical tests were as follows: simultaneous electromyography of the affected and unaffected tibialis anterior and rectus femoris muscles bilaterally in voluntary isometric contraction; the Six-Minute Walk Test; the Stair-Climb Test; and the Timed Get-Up-and-Go Test. The data were analyzed by independent and paired t tests and by analysis of covariance. ResultsThere was no evidence of effects on the group and time interaction relative to variables affected side rectus femoris, unaffected side rectus femoris, affected side tibialis anterior, unaffected side tibialis anterior, and the Stair-Climb Test (P > 0.05). There was evidence of effects on the group interaction relative to variables Six-Minute Walk Test and Timed Get-Up-and-Go Test (P < 0.05). ConclusionsWhole-body vibration contributed little to improve the functional levels of stroke patients.
Adriana Silva, Andreia Silva, Miqueline Dias, Ruanito Calixto, Beatriz Martinez, Donizeti Honorato, and Geraldo Fernandes
Mark Allen Group
Background/aimMotor function impairment is a frequent problem in stroke patients and many questions remain about the application of vibration therapy for neurological patients. This study aims to verify the effects of vibration training on lower limb function in patients affected by stroke.MethodsThis was a clinical, prospective study. The study's subjects were selected via a convenience sample from Samuel Libanio Hospital's Physical Therapy and Neurology Out-patient clinics in Pouso Alegre, Brazil. The sample comprised of 27 individuals presenting with spastic hemiparesis due to stroke. Participants received whole body vibration training at a frequency of 50 Hz and 2 mm amplitude, for 8 weeks. In the first 4 weeks, participants underwent a series of four 60-second periods of vibration in the following positions: in orthostatic posture, first with the knees semi-flexed at 30°, then with the knees semi-flexed at 90°, and then with the affected knee semi-flexed at 30°, and finally in orthostatic posture again with the knees semi-flexed at 30°. In the last 4 weeks, we doubled the number of series of vibration, but used the same positions. The six minute walking test (6MWT), the stair climbing test (SCT) and the timed get up and go test (TUG) were applied to evaluate the lower members' motor function. The tests were applied before and after the intervention, as well as after a follow-up, which was performed 1 month later.ResultsThis study found statistical differences in the 6MWT (P=0.00), SCT (P=0.00), and TUG (P=0.00), indicating improvement in the participants' lower limbs motor function.ConclusionVibration therapy can contribute to the improvement of the lower limbs' motor function in patients affected by stroke.